Trans-Excision-Splicing Ribozyme and Methods of Use
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چکیده
منابع مشابه
Tetrahymena thermophila and Candida albicans Group I intron-derived ribozymes can catalyze the trans-excision-splicing reaction
Group I intron-derived ribozymes can catalyze a variety of non-native reactions. For the trans-excision-splicing (TES) reaction, an intron-derived ribozyme from the opportunistic pathogen Pneumocystis carinii catalyzes the excision of a predefined region from within an RNA substrate with subsequent ligation of the flanking regions. To establish TES as a general ribozyme-mediated reaction, intro...
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The Tetrahymena trans-splicing ribozyme can edit RNA in a sequence-specific manner, but its efficiency needs to be improved for any functional rescues. This communication describes a simple method that uses a bacterial enzyme beta-lactamase to report trans-splicing activity of Tetrahymena ribozyme in single living mammalian cells by fluorescence microscopy and flow cytometry. This enzyme-based ...
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The detection of mRNA expression in vivo can reveal essential information about basic biology and disease processes. Current methods primarily involve the use of labeled (with fluorophores or radioactive isotopes) antisense oligomers, based on a one-to-one receptor–ligand type of interaction without robust signal amplification, such as molecular beacons. Given that the copy number of a particul...
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PURPOSE Our previous studies suggested that human telomerase reverse transcriptase (hTERT) RNA-targeting trans-splicing ribozyme could be a useful tool for cancer gene therapy. Here, we investigated whether adenoviruses harboring this ribozyme can be systemically delivered to mice, and whether they selectively mark tumors expressing hTERT and sensitize them to ganciclovir treatments. EXPERIME...
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Natural intron-like self-splicing ribozymes have been re-engineered to trans-splice two arbitrary RNA pieces together. This capability has potential to be tremendously useful for the synthetic biologist. We propose analyzing the suitability of these ribozymes as a tool for engineering biology by adapting trans-splicing ribozymes for use in measuring, debugging, patching, and building biological...
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